Shiitake Mushroom Plate Culture

NSLE2 - Acorn (Lentinula edodes)
$74.99

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Species & Strain

Start your shiitake project with a clean, versatile culture source. The Shiitake Mushroom Plate Culture contains Lentinula edodes mycelium on an agar plate, giving intermediate growers a practical foundation for expanding shiitake cultures and producing their own spawn.

Plate cultures are useful for maintaining a working culture and transferring vigorous mycelium into new media. Use this culture to make master bags, inoculate sterilized grain, prepare liquid culture, or create additional agar plates for continued propagation.

Each shiitake plate is made to order in North Spore’s in-house laboratory using medical-grade materials and sterile culture techniques. This controlled production approach supports consistent handling from the lab to your workspace.

Because agar work requires careful sterile technique, this product is best suited to growers who already have basic lab supplies and experience with transfers. A still-air box or flow hood, sterile tools, and properly prepared media are recommended for successful expansion.

Shiitake grows more deliberately than many fast-colonizing species, making clean culture work especially important. Once expanded, Lentinula edodes culture can be used for indoor spawn production or transferred into appropriate hardwood-based substrates.

Store the plate as directed upon arrival and minimize exposure during transfers. With proper technique, this Shiitake Mushroom Plate Culture provides a dependable starting point for building a larger, customized cultivation system.

NSLE1 - 207 Shiitake

Lentinula edodes. Our favorite commercial strain for indoor shiitake block production. This strain fruits best at colder temperatures and tends to produce mushrooms with large caps and smaller stems. Our culture is direct from Belgium and retains the highly desired characteristics of this strain. Yield averages are .75-1.5 lb first flush off of 5 lb supplemented sawdust blocks. 

NSLE2 - Acorn Shiitake

Lentinula edodes. A variation on commercial indoor shiitake genetics. We've had similar results from this strain as from our '207' strain listed above. We like having multiple varieties of the same species to ensure genetic diversity and reduce the odds of crop failure.

NSLE3 - Log Shiitake

Lentinula edodes. Fast spawn run, heavy first flushes, and quick log recovery after fruiting.

NSLE4 - Warm Shiitake

Lentinula edodes. Warm weather strain that responds well to force fruiting. Grows large, plump mushrooms. 

NSLE5 - Cool Shiitake

Lentinula edodes. A highly productive, cool season fruiter that yields heavier in the spring.  

NSLE9 - Aloha Shiitake

Lentinula edodes. Reilable strain, even if the growing conditions and substrate are not perfect. Can tolerate a wider range of temperatures than most shiitake. Produces medium sized caps. 

Frequently Asked Questions

We recommend absolute beginners start with our Spray & Grow Kits, which make growing mushrooms at home super easy.

We want you to have the best possible experience with every North Spore product and guarantee free replacements within 30 days of receipt for any damaged, contaminated, or unusable product.

Absolutely! Mushroom cultivation is a great activity to do with the whole family, whether you'd like to grow indoors or outdoors. If you're new to mushrooms, we recommend starting with a Spray & Grow Kit or a Plant & Grow Kit – both make it easy to grow fresh, organic mushrooms at home.

We are vertically integrated — from culture work all the way to spawn and substrate production — ensuring a quality end product. And our staff mycologists keep up to date on all best practices in our field. Additionally, we have a dedicated QAQC team that tests every batch of grain and substrate before it’s inoculated.

Cluster of brown-capped mushrooms with long, light-colored stems against a white background.

Your Trusted Source for Premium Mushroom Growing Supplies

Whether you’re growing mushrooms at home, in your garden, or at a commercial scale, we offer everything you need to cultivate success. We start with the highest-quality OMRI-listed ingredients, mix them in our state-of-the-art facility, and inoculate them in our custom-built lab, so you can rest easy knowing your product will yield the best results.

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