We recommend wearing latex gloves and working inside a still air box (like the NocBox) or in front of a flow hood. If you do not have access to either, a well-cleaned space can be used, but contamination is more likely.

Choose a location with little air movement, shut windows and doors, turn off any heating or cooling units, and choose a work surface you can sanitize with isopropyl alcohol.

Begin by sanitizing your work surface with 70% isopropyl alcohol. Let sit on surfaces until dry or for several seconds before wiping dry with a clean paper towel. 

• If the liquid culture or spore syringe doesn’t look uniform, you may shake it for a few seconds to disperse the mycelium or spores.
• Inject 2-5 cc's of a spore syringe or liquid culture into the jar. (Needles come sterile in their packaging, but subsequent use of a culture or spore syringe will require re-sterilization of the needle.)
• Sterilize the needle with alcohol or flame sterilize with an alcohol burner or lighter. If using a flame to sterilize, the tip of the needle should be heated until it glows red and then allowed to cool before injection.

⚠️ WARNING: Do not use an open flame inside a still-air box. Agitate the jar by shaking it for 20-30 seconds. This will help to disperse the spores or culture throughout the media.

Complete instructions can be found in the instruction manual.

• Allow your liquid culture to come to room temperature before use.
• Agitate the jar before you draw up the liquid culture.
• Insert your syringe into the self-healing injection port.
• Tip the jar towards the syringe, but do not let the liquid touch the filter patch.
• If the syringe gets clogged or you draw air into your syringe when you are withdrawing the liquid, keep the syringe in the injection port, push the liquid back into the jar, and withdraw the correct amount.
• After use, return your jar to refrigeration and use it within four months.

Complete instructions can be found in the instruction manual.

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