To inoculate the grain bag, simply inject your spore or culture syringe through the port in a sterile location. We always recommend clean hands and latex gloves to reduce the introduction of contaminants.

It is best practice to wait a minimum of 1 week after arrival before injecting grain bags to ensure they were not compromised during transit. Rest assured that your purchase is 100% guaranteed. If your bag was compromised, we will replace it. 

We recommend using 2.5-5ccs per 3lb injection port bag. We do not typically recommend using an entire syringe in one bag. Doing so adds excessive moisture which can create over saturation in the bag. This over-saturation can pool and is prone to being anaerobic and the mycelium will have a hard time colonizing.

Allow your bag to colonize in a cool location out of direct sunlight. Temperature for incubation shouldn't exceed 75 degrees F or dip below 55 degrees F. After a couple weeks, you should start to see mycelium growing on the grain. 

You'll notice the white mycelium growing outward from the injection point. When it has grown three to four inches you should, without opening the bag, carefully break up the colonized grain and mix it into the uncolonized grain. This will dramatically speed up the colonization process.

When the bag is completely white with mycelium your spawn is ready to use. This may take a couple weeks to a month or more depending on the species and vigor of your culture.

Grain bags should be stored at room temperature out of direct sunlight. No refrigeration is necessary. They can also be stored for several months before use.

Our grain bags are sterilized in a commercial-grade autoclave. Sterility is verified using biological indicators and temperature sensors.