In the video below, William Padilla-Brown (founder of MycoSymbiotics) walks through the cultivation process of Cordyceps militaris, a mushroom known for its many medicinal properties. He covers proper technique for jar cultivation using a pressure cooker, in addition to cultivation with a rice cooker or Instant Pot.
What you'll need:
Jar Tech
Poly-fil
12 pint canning jars
Mixing spoon
Scale scoop
Mixing bowl
Pressure cooker
Latex gloves
70% isopropyl
Paper towels
Cordyceps liquid culture
Bin Tech
Scale scoop
Mixing spoon
Mixing bowl
Shoebox size plastic bin
Parafilm
Rice cooker / Instant Pot
Latex gloves
70% isopropyl
Paper towels
Cordyceps liquid culture
Jar Tech
Poly-fill
12 pint jars
Mixing spoon
Scale scoop
Mixing bowl
Pressure cooker
Gloves
70% isopropyl
Paper towels
Cordyceps culture
Bin Tech
Scale scoop
Mixing spoon
Mixing bowl
Plastic bin
Parafilm
Rice cooker/instapot
Gloves
70% isopropyl
Paper towels
Cordyceps culture
Creating your nutrient broth
For both jar and bin cultivation, you will need to start by making a nutrient broth. Nutrient broth is used to hydrate your brown rice, or "matrix" for the mushroom mycelium to grow on. Cordyceps naturally grow on insect larva, so your brown rice and broth substrate will be a nutrient-rich substitute.
Ingredients for nutrient broth:
1 gallon coconut water
10 grams tapioca starch
5 grams magnesium sulfate
10 grams multivitamins (crushed)
19 grams nutritional yeast or soy peptone
11 grams kelp powder
3.5 grams gypsum
Combine dry ingredients: nutritional yeast or soy peptone, tapioca starch, magnesium sulfate, kelp powder, crushed multivitamins, and gypsum, in a mixing bowl. Add coconut water and mix thoroughly. Extra broth can be stored in the fridge for up to one week.
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